The objective of Experiment 1 was to determine the effect of bovine follicular fluid (bFF) on nuclear maturation. Treatment A (Control) oocytes were stained with Hoechst-33342 immediately after aspiration from follicles, whereas, oocytes in Treatment B were held in bFF for 12 hours at 38¢ªC and then stained to determine nuclear status. No significant difference was detected between treatment groups. Results indicate that bFF inhibits resumption of meiosis. The objective of Experiment 2 was to determine the effect of bFF on embryonic development. Oocytes in Treatment A (Control) were placed into in vitro maturation (IVM) for 22 hours followed by in vitro fertilization (IVF). Oocytes in Treatment B were held in bFF for 12 hours at 22¢ªC, followed by IVM and then subjected to IVF. Significantly more (P<0.0001) oocytes cleaved, developed into blastocysts and hatched in Treatment A compared with Treatment B. Results indicate that a 12-hour holding period in bFF does not promote normal embryonic development. The objective of Experiment 3 was to determine the effect of decreased time and concentration of bFF on embryonic development. Treatment A (Control) oocytes were placed into IVM followed by IVF. Oocytes in Treatment B were held in bFF, oocytes in Treatment C were held in Lactated Ringer¡¯s Solution (LRS) and oocytes in Treatment D were held in a combination of bFF and LRS for 6 hours at 22¢ªC, followed by IVM then by IVF. No significant difference was detected between Treatments A and B when analyzing cleavage, blastocyst formation and hatching rates. However, significantly fewer (P<0.0001) embryos reached these stages of development in Treatments C and D. Nevertheless, there were significantly more embryos that developed to the blastocyst stage in Treatment D compared with Treatment C. Decreasing the amount of time that oocytes were held in bFF proved to be beneficial in supporting in vitro embryo production (IVP). These findings could be advantageous when attempting to rescue valuable gametes from deceased females.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-03242004-175322 |
| Date | 29 March 2004 |
| Creators | Klumpp, Angela Marie |
| Contributors | Robert Godke, Dale Paccamonti, Stanley Leibo |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-03242004-175322/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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