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Preparation and characterization of peptide-directed polyclonal antibodies against angiotensin receptors.

Anita K.L. Yiu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 93-112). / Acknowledgement --- p.i / List of Abbreviations --- p.ii / Abstract --- p.iv / Table of Contents --- p.vi / Chapter CHAPTER 1. --- Introduction / Chapter 1.1 --- The Renin-Angiotensin System (RAS) --- p.1 / Chapter 1.2 --- Physiology and Pathophysiology of Angiotensin --- p.3 / Chapter 1.3 --- Angiotensin Receptors / Chapter 1.3.1 --- Heterogeneity among Angiotensin Receptors --- p.10 / Chapter 1.3.2 --- Differential Distribution of Subtypes --- p.13 / Chapter 1.3.3 --- Molecular Structure of Subtypes --- p.15 / Chapter 1.3.4 --- Signal Transduction Mechanism --- p.20 / Chapter 1.3.5 --- Physiological Functional Correlates --- p.21 / Chapter 1.4 --- Aim of Study --- p.23 / Chapter CHAPTER 2. --- Preparation of Polyclonal Antibodies Against Angiotensin Receptors / Chapter 2.1 --- Introduction --- p.25 / Chapter 2.2 --- Methods / Chapter 2.2.1 --- Preparation of antisera / Chapter 2.2.1.1 --- Preparation of peptide conjugates --- p.25 / Chapter 2.2.1.2 --- Protein determination --- p.27 / Chapter 2.2.1.3 --- Immunization of rabbits with peptide conjugates --- p.27 / Chapter 2.2.1.4 --- Collection of rabbit sera --- p.28 / Chapter 2.2.1.5 --- Affinity purification of antisera --- p.28 / Chapter 2.2.2 --- Enzyme-linked immunosorbent assay (ELISA) / Chapter 2.2.2.1 --- Titer determination --- p.29 / Chapter 2.2.2.2 --- Specificity determination --- p.30 / Chapter 2.3 --- Results / Chapter 2.3.1 --- Preparation of antisera --- p.30 / Chapter 2.3.2 --- Affinity purification of antisera --- p.30 / Chapter 2.3.3 --- ELISA / Chapter 2.3.3.1 --- Titer determination --- p.31 / Chapter 2.3.3.1.1 --- Thy-AT1 antiserum --- p.31 / Chapter 2.3.3.1.2 --- Thy-AT2 antiserum --- p.32 / Chapter 2.3.3.2 --- Specificity determination --- p.32 / Chapter 2.3.3.2.1 --- Thy-AT1 antibodies --- p.32 / Chapter 2.3.3.2.2 --- Thy-AT2 antibodies --- p.49 / Chapter 2.4 --- Discussions --- p.49 / Chapter CHAPTER 3. --- Application of Thy-AT1 Antiserumin Western Blot / Chapter 3.1 --- Introduction --- p.52 / Chapter 3.2 --- Methods / Chapter 3.2.1 --- Preparation of protein samples --- p.52 / Chapter 3.2.2 --- Protein determination --- p.53 / Chapter 3.2.3 --- SDS-PAGE --- p.53 / Chapter 3.2.3 --- Western blot --- p.54 / Chapter 3.2.5 --- Immunoblotting --- p.54 / Chapter 3.3 --- Results --- p.55 / Chapter 3.4 --- Discussions --- p.58 / Chapter CHAPTER 4. --- Evaluation of Pancreatic Response to Angiotensin II / Chapter 4.1 --- Introduction --- p.61 / Chapter 4.2 --- Methods / Chapter 4.2.1 --- Perfusion of pancreas --- p.62 / Chapter 4.2.2 --- Assay of amylase activity --- p.64 / Chapter 4.2.3. --- Calculations --- p.64 / Chapter 4.3 --- Results --- p.65 / Chapter 4.4 --- Discussions --- p.65 / Chapter CHAPTER 5. --- Application of Purified Thy-AT2 Antibodies in immunohistochemical studies / Chapter 5.1 --- Introduction --- p.74 / Chapter 5.2 --- Methods / Chapter 5.2.1 --- Preparation of adrenal sections --- p.75 / Chapter 5.2.2 --- Light-microscopic immunohistochemical study --- p.76 / Chapter 5.3 --- Results / Chapter 5.4 --- Discussions / Chapter CHAPTER 6. --- General Discussions --- p.84 / References --- p.93 / Appendix / Chapter A. --- Materials --- p.113 / Chapter B. --- Buffer Compositions --- p.121

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322586
Date January 1996
ContributorsYiu, Anita K. L., Chinese University of Hong Kong Graduate School. Division of Biochemistry.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, viii, 124 leaves : ill. (some mounted) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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