The novel hypothesis that antimicrobial growth promoters (AGP) function by
modulating the mammalian immune system was tested. Sampling methods to
characterize the mucosa-associated microbiota of the murine intestine by terminal
restriction fragment length polymorphism (T-RFLP) analysis indicated that direct plug
extraction was superior to wash methods. Using T-RFLP analysis, non-therapeutic
administration of chlortetracycline (CTC) and sulfamethazine to beef cattle did not affect
the composition of bacterial communities associated with intestinal mucosa and in
digesta, with exception of those associated with mucosa of the proximal jejunum.
Similarly, oral administration of non-therapeutic concentrations of CTC did not affect the
mucosa-associated microbiota of the murine intestine. Oral administration of nontherapeutic
concentrations of CTC prevented weight loss, reduced pathologic changes,
modulated transcription levels of inflammatory cytokines in C. rodentium-infected mice,
and did not consistently affect the colonic microbiota. These findings support the
hypothesis that AGP primarily function by modulating the intestinal immune system. / xiv, 160 leaves ; 29 cm
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:ALU.w.uleth.ca/dspace#10133/2627 |
| Date | January 2010 |
| Creators | Costa, Estela, University of Lethbridge. Faculty of Arts and Science |
| Contributors | Inglis, G. Douglas |
| Publisher | Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2010, Arts and Science, Department of Biological Sciences |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_US |
| Detected Language | English |
| Type | Thesis |
| Relation | Thesis (University of Lethbridge. Faculty of Arts and Science) |
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