FundaÃÃo de Amparo à Pesquisa do Estado do Cearà / Os acidentes ofÃdicos de serpentes representam um sÃrio problema de SaÃde PÃblica nos paÃses tropicais, tanto pela freqÃÃncia com que ocorrem e/ou pela morbi-mortalidade que ocasionam. As serpentes do gÃnero Crotalus estÃo representadas no Brasil pela espÃcie Crotalus durissus, a qual se divide em seis subespÃcies. Nosso trabalho teve como objetivo avaliar os efeitos dos venenos das serpentes Crotalus durissus cascavella originadas do estado do Cearà (Cdcc) e MaranhÃo (Cdcm); Crotalus durissus collilineatus (Cdcol); Crotalus durissus ruruima (Cdru) e suas fraÃÃes, Crotoxina (CTXru) e Fosfolipase A2 (PLA2ru), nos processos biolÃgicos de espraiamento celular, fagocitose, atividade fungicida e alteraÃÃes hematolÃgicas. Camundongos Swiss, machos, foram inoculados por via intraperitonial com os venenos descritos acima, nas doses de 120, 50, 27, 20 (venenos) e 10Âg/Kg (fraÃÃes), respectivamente. Duas horas apÃs inoculaÃÃo foram coletadas amostras de sangue do plexo orbital e o exsudato peritonial. A anÃlise estatÃstica utilizada foi o teste t de Student com significÃncia de 95%. Os animais tratados foram comparados com o grupo controle (inoculados com salina 0,9%). Cdcm e a CTXru causaram as maiores alteraÃÃes no eritrograma. 37,5% dos eritrÃcitos apresentaram morfologia macrocÃtica e microcÃtica; 25,5% hipocrÃmia; 25% com anisocitose e presenÃa de policromasia. Foram observados 16,8% de corpÃsculos de Howell Jolly. A contagem global de leucÃcitos foi reduzida significantemente apÃs administraÃÃo do Cdcc (82,9%), Cdcm (70,1%) e Cdru (83,8%). A celularidade foi alterada depois da inoculaÃÃo de Cdcc, Cdru e CTXru, em todos os tipos de cÃlulas. A contagem global de cÃlulas do peritÃnio aumentou apÃs inoculaÃÃo de Cdcc, Cdcol, Cdru e a CTXru. Em adiÃÃo, o macrÃfago foi à cÃlula predominante na contagem diferencial de cÃlulas peritoniais, contudo, somente a Cdcol apresentou significÃncia estatÃstica para macrÃfago (62,3%). Foi encontrada reduÃÃo significativa do espraiamento celular depois da administraÃÃo de todos os venenos variando de 52,7 a 65,7%. A fagocitose foi estatisticamente reduzida pela Cdcc nos perÃodos de 30, 60, 90 e 120 minutos. Cdru reduziu a fagocitose apenas em 30, 60 e 120 minutos, Cdcm em 30 e 90 minutos e CTXru nos tempos de 60 e 120 minutos. A Cdcol, e a CTXru mostraram significÃncia na atividade fungicida contra C. albicans nos perÃodos de 30, 60, 90 e 120 minutos, mas a Cdcc mostrou resultado similar em 60, 90 e 120 minutos. Conclui-se que o veneno interfere diferentemente na resposta hematolÃgica e funcional. Em adiÃÃo pode-se postular que os macrÃfagos foram responsÃveis por estas alteraÃÃes. Estudos futuros deverÃo ser realizados na perspectiva da identificaÃÃo de provÃvel aÃÃo fungicida de venenos ofÃdicos e suas fraÃÃes / Venomous snake accidents represent a serious public health problem in tropical countries, as much as for their frequency of occurrence and/or morbidity and mortality that they caused. In Brazil, the genus Crotalus comprise only one species, termed Crotalus durissus, which is divided into six subspecies. The aim of our study was to evaluate the effects promoted by venoms of Crotalus durissus cascavella, originated from the States of Cearà (Cdcc) and MaranhÃo (Cdcm); C. durissus collilineatus (Cdcol); C. durissus ruruima (Cdru) and its isolated components, such as crotoxin (CTXru) and phospholipase A2 (PLA2ru), in the biological processes of cellular spreading, phagocytosis, hematological alterations and antifungal activity. Male Swiss mice were inoculated intraperitoneally with the venom doses of 120, 50, 27, 20, 10 and 10 Âg/Kg, respectively to the snakes described above. After two hours of inoculation blood samples and exudate were collected from orbital plex and peritoneum, respectively. Statistical evaluation was performed using Student-T test with significance level set at 95%. We compared the treated animals with a control group, where animals were inoculated with saline 0.9%. Cdcm and CTXru caused the most severe alterations in the erythrogram. We noticed that 37.5% of the erythrocytes showed macrocytic and microcytic morphology; 25.5% were hipocromic; 25% showed anisocytosis and the presence of polycromasia. We also found Howell Jolly bodies in 16.8% of the examined erythocytes. The total counting of leukocytes was reduced statistically after administration of Cdcc (82.9%), Cdcm (70.1%) and Cdru (83.8%). Cellularity was altered after the inoculation of Cdcc, Cdru and CTXru for all evaluated cells. We noticed a statistic increase of peritoneum total cells caused by Cdcc, Cdcol, Cdru and CTXru. In addition, macrophage was the most predominant cell after peritoneum differential cell counting. However, only Cdcol showed a statistic increase of macrophages (62.3%). We found significant reduction of cellular spreading after administration of all venoms ranging from 52.7 to 65.7%. Phagocytosis was statistically reduced by Cdcc in the periods of 30, 60, 90 and 120 minutes. However, Cdru reduced phagocytosis only at 30, 60 and 90 minutes, Cdcm decreased phagocytosis at 30 and 90 minutes and CTXru in the periods of 60 and 120 minutes. Cdcol and CTXru showed significant fungicide activity against C. albicans in the periods of 30, 60, 90 and 120 minutes, but Cdcc showed similar results at 60, 90 and 120 minutes. We conclude that distinct venoms interfered differently in the intensity of each functional and hematological response. In addition, we postulate that macrophages maybe partially responsible for these alterations. Further studies should be evaluated for the use of venoms as fungicides
| Identifer | oai:union.ndltd.org:IBICT/oai:www.teses.ufc.br:433 |
| Date | 27 November 2006 |
| Creators | IÃda Pereira de Souza |
| Contributors | Helena Serra Azul Monteiro, Krishnamurti de Morais Carvalho, RomÃlia Pinheiro GonÃalves Lemes, ArlÃndia Cristina Lima Nobre de Morais, Marcos Hikari Toyama |
| Publisher | Universidade Federal do CearÃ, Programa de PÃs-GraduaÃÃo em Farmacologia, UFC, BR |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFC, instname:Universidade Federal do Ceará, instacron:UFC |
| Rights | info:eu-repo/semantics/openAccess |
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