Wan, Chung Tin. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 126-139). / Abstracts in English and Chinese. / Thesis / Assessment Committee --- p.i / Acknowledgement --- p.ii / Abstract (English) --- p.iii / Abstract (Chinese) --- p.v / List of Abbreviations --- p.vii / List of Tables --- p.ix / List of Figures --- p.x / Chapter Chanter 1 --- Introduction / Chapter 1.1. --- Overview of cancer --- p.1 / Chapter 1.1.1 --- Breast cancer --- p.1 / Chapter 1.1.2 --- Hepatocellular carcinoma (HCC) --- p.2 / Chapter 1.2. --- Role of natural products in the fight against cancer --- p.3 / Chapter 1.2.1 --- Introduction of Astragalus membranaceus --- p.4 / Chapter 1.2.2 --- Introduction of Acanthopanax senticosus --- p.9 / Chapter 1.2.3 --- Introduction of Grifola Frondosa --- p.13 / Chapter 1.3. --- Apoptosis and cancer --- p.17 / Chapter 1.3.1 --- Caspases --- p.20 / Chapter 1.3.2 --- Intrinsic apoptotic pathway --- p.21 / Chapter 1.3.3 --- Extrinsic apoptotic pathway --- p.23 / Chapter 1.3.4 --- Execution of apoptosis --- p.26 / Chapter 1.4 --- Cell cycle and cancer --- p.27 / Chapter 1.5 --- Objective of this project: --- p.29 / Chapter Chapter 2 --- Materials and Methods / Chapter 2.1 --- Materials: --- p.30 / Chapter 2.1.1 --- Culture medium --- p.30 / Chapter 2.1.2 --- Buffers and reagents --- p.31 / Chapter 2.1.3 --- Herbs --- p.34 / Chapter 2.2 --- Methods --- p.35 / Chapter 2.2.1 --- Cell cultures --- p.35 / Chapter 2.2.1.1 --- Cell lines --- p.35 / Chapter 2.2.1.2 --- Cell culture techniques --- p.36 / Chapter 2.2.1.3 --- Cryopreservation of cell lines --- p.36 / Chapter 2.2.2 --- Proliferation assay --- p.37 / Chapter 2.2.2.1 --- MTT assay --- p.37 / Chapter 2.2.2.2 --- Isolation of PBMC --- p.38 / Chapter 2.2.2.3 --- XTT --- p.39 / Chapter 2.2.3 --- DNA fragmentation --- p.39 / Chapter 2.2.4 --- Flow cytometry --- p.41 / Chapter 2.2.4.1 --- Detection of mitochondrial membrane depolarization by the use of JC-1 --- p.41 / Chapter 2.2.4.2 --- Annexin-V-FITC PI labeling of apoptotic cells --- p.42 / Chapter 2.2.4.3 --- Cell cycle analysis --- p.43 / Chapter 2.2.5 --- Western blotting --- p.43 / Chapter 2.2.5.1 --- Total protein extraction --- p.44 / Chapter 2.2.5.2 --- Determining protein concentration --- p.44 / Chapter 2.2.5.3 --- SDS-PAGE --- p.45 / Chapter 2.2.5.4 --- Electroblotting --- p.45 / Chapter 2.2.5.5 --- Probing --- p.46 / Chapter 2.2.5.6 --- ECL --- p.46 / Chapter 2.2.6 --- Preparation of herbal extracts --- p.47 / Chapter 2.2.6.1 --- Preparation of extract of Astragalus membranaceus and Grifola frondosa --- p.47 / Chapter 2.2.6.2 --- Preparation of Acanthopanax senticosus aqueous extract --- p.47 / Chapter 2.2.6.3 --- Partition of Acanthopanax --- p.47 / Chapter 2.2.6.4 --- Column purification of ethyl-acetate fraction --- p.48 / Chapter 2.2.6.5 --- Analytical thin layer chromatography --- p.49 / Chapter 2.2.7 --- Statistical Analysis --- p.50 / Chapter Chanter 3 --- Results / Chapter 3.1 --- Extractions --- p.51 / Chapter 3.2 --- Anti-proliferative effect of herbal extracts on cancer cell lines --- p.51 / Chapter 3.3 --- Partition of Acanthopanax methanol extract --- p.56 / Chapter 3.4 --- Anti-proliferative effect of Acanthopanax partition fractions on breast cancer cells --- p.59 / Chapter 3.5 --- Column chromatography of ethyl acetate fraction --- p.59 / Chapter 3.6 --- Anti-proliferative effect of various sub-fractions on breast cancer cells --- p.64 / Chapter 3.7 --- Effect of sub-fractions on PBMC proliferation --- p.76 / Chapter 3.8 --- Kinetic study of anti-proliferative effect of Fc --- p.76 / Chapter 3.9 --- Flow cytometric analysis --- p.79 / Chapter 3.91 --- JC-1 staining --- p.79 / Chapter 3.92 --- Annexin-P1 labeling --- p.79 / Chapter 3.93 --- Cell cycle analysis --- p.80 / Chapter 3.10 --- DNA fragmentation assay --- p.88 / Chapter 3.11 --- Western blotting --- p.91 / Chapter Chanter 4 --- Discussion --- p.99 / Chapter Chapter 5 --- Conclusion --- p.124 / References --- p.126
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326547 |
| Date | January 2008 |
| Contributors | Wan, Chung Tin., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xiv, 139 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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