Abstract
C-Phycocyanin (C-PC), a water-soluble protein pigment, is one of the major constituents of Spirulina platenisi,. Which is a blue green algae and used in many countries as dietary supplements.The C-PC used in present study is a phycobiliprotein, and consist of two distinguishable protein subunits designated as £\ and £] subunits.
The aim of this study was to screen pharmacological effects including analgesic, anti-inflammatory and hepatoprotective effects of C-PC in an animal model. Concomitantly, the antioxidant effect of C-PC was measured to confirm its free radical scavenger activity.
The analgesic effect of C-PC measured by tail-flick test shown the latency (sec) of control mice is approximately 4-5 sec. After administration different doses of C-PC (0.1, 1.0, 10 mg/kg), the latency resulted in a dose-dependent phenomenon and prolong from 4 sec to 4.5, 5.6, and 6.9 sec, respectively.
The anti-inflammatory effect of C-PC was analyzed in the £f ¡Vcarrageenan injected rat. The hind paw edema percentage change by injection of different doses (0.1~1.0 mg/kg) of C-PC, our results have indicated that C-PC possessed a dose-dependent inhibitory effect on carrageenan-induced edema formation.
Intraperitoneal (i.p.) injection of different dose (0.01,0.1 1, 1.0 mg/kg) of C-PC on CCl4 (0.3 ml/kg) challenged rats have indicated that C-PC possessed a significant hepatotoprotective effect and significant decrease the serum levels of glutamate pyruvate transaminase (SGPT) and serum glutamate oxyloacetic transaminase (SGOT).
Antioxidant effect of C-PC was measured by both xanthine oxidase method, and cytochrome c method. The IC50 of C-PC measured by xanthine oxidase method is 7.23 ¡Ó0.21 mg/ml. The IC50 value of C-PC estimated by cytochrome c method is approximately 6.1¡Ó0.74 mg/ml.
Moreover, the direct active oxygen (¡PO2-) and hydrogen oxide (¡POH) radical scavenging (SOD-like) activity was also confirmed by using advance electron spin resonance (ESR) method. We used the spin-trapping technique to evaluate free radical scavenging ability of C-PC. The IC50 (¡PO2- ) and (¡POH) scavenging activities are 1.08 ¡Ñ 103 unit/g and 7.11 unit/g.
Our results have shown the superoxide radical (¡POOH) scavenging activity of C-PC is approximately 3.28 mg/ml. By contrast, IC50 of the hydroxyl radical (¡POH) scavenging activity of C-PC is 4.75 mg/ml.
Human hepatoma cells (HepG2 and 2.2.15 cells) and rat glioma C6 cells were cocultured with different concentration of C-PC (1.0, 10 and 20 £gg/ml)for 72 hours, the cell cycle regulation of C-PC was measured by flow cytometer. We found the C-PC possessed a G0/G1 phase-arrest effect on all of the three kind cancer cell line. Whereas, the cell cycle modulations of C-PC are more significant in human hepatoma cells.
In conclusion, both our pharmacological screening tests and antioxidant bioactivity assay have indicated that C-PC is a potential antitumor, anti-inflammatory and analgesic agent.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0916102-140037 |
| Date | 16 September 2002 |
| Creators | Yung-Hong, Wong |
| Contributors | Liu Li-Lian, none, none, none |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0916102-140037 |
| Rights | not_available, Copyright information available at source archive |
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