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Histochemical Characterization of Lymphocytes in Preleukemic and Leukemic AKR Mice

The AKR strain of mice have a genetic trait for spontaneous development of lymphocytic leukemia. In this study, leukemic mice were found to have significantly larger (p<0.01) thymuses and spleens than preleukemic mice. The enlarged leukemic tissues were densely packed with a light staining cell, with a hollow-appearing nucleus. Tissues from preleukemic mice were observed to be infiltrated with a smaller, darker-staining lymphocyte. Fluorescent antibody staining was done on preleukemic and leukemic tissues, using three antisera against murine lymphocyte theta antigen, and an antiserum against murine IgG. Significantly brighter fluorescence, (p <0.05) with theta-specific antisera, was found in leukemic thymuses,spleens, and kidneys than in the same preleukemic tissues. Leukemic tissues had significantly brighter fluorescence (p <0.05) than preleukemic tissues with IgG antiserum.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc503886
Date05 1900
CreatorsMichnoff, Carolyn A.
ContributorsHatten, Betty A., Norton, S. J., Kester, Andrew S.
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatv, 117 leaves : ill., Text
RightsPublic, Michnoff, Carolyn A., Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved.

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