This study examined the use of phage therapy against Pseudomonas aeruginosa strain PA14 biofilms. Part 1: 24 and 96h PA14 biofilms grown in microplates were phage treated and bacterial biomass was quantified using crystal violet staining. Part 2: 24 and 96h PA14 biofilms grown in prepared root canals of human mandibular incisors were treated with phages and intra-canal samples using paper points and round burs were taken to assess phage and bacterial counts. Part 1: Two phages (JBD4 and JBD44a) were used. Treatment with phages produced significant reduction in the mean percentage of biomass in 24h (p<0.05) and 96h (p=0.08) biofilms. Part 2: In 24 and 96h biofilms in a root canal model, no significant difference was found in colony forming units after phage treatment (p>0.05). Phage application significantly reduced the biomass of 24 and 96h PA14 biofilms grown on microplates, but did not in the extracted tooth models.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/33491 |
| Date | 26 November 2012 |
| Creators | Phee, Alysen Leigh |
| Contributors | Basrani, Bettina |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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