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Previous issue date: 2006-08-18 / Hormone therapy is an important tool in the treatment of breast cancer and tamoxifen represents one of the most important drugs used in this type of treatment. Recently other drugs based on the inhibition of aromatase had been
developed, this enzyme is responsible for the synthesis of estrogenic esteroids from the androgenic ones. The objective of this study would be the development of a quantitative cytological model of murine estral analysis that allowed the
characterization of different hormone drugs effect over vaginal epithelium. The technique of monochromatic staining with Evans blue (C.I. 23860) showed to be efficient in the qualitative and quantitative classification of the cycle. It had been observed differences in the cytological standard of animals submitted to the studied drugs; tamoxifen presented a widening of phases of lesser maturation (diestrais), while anastrozole and exemestane increased the duration of the
phases of larger maturation (estrais). The data were analysed through a cubical non linear regression (spline) which allowed a better characterization of the drugs, suggesting a proper cytological profile to the antagonism of the estrogen receptor
(tamoxifen), aromatase competition (anastrozole) and inhibition of the enzyme (exemestane) / A hormonioterapia ? um importante recurso no tratamento do c?ncer de mama e o tamoxifeno representa o f?rmaco mais empregado neste tipo de tratamento. Recentemente foram desenvolvidos outros f?rmacos baseados na inibi??o da
aromatase, enzima respons?vel pela s?ntese de ester?ides estrog?nicos a partir de androg?nios. O objetivo deste estudo seria o desenvolvimento de um modelo citol?gico quantitativo de analise estral murina que permitisse a caracteriza??o
dos efeitos farmacol?gicos de diferentes hormonioter?picos sob epit?lio vaginal. A t?cnica de colora??o monocrom?tica com Azul de Evans (C.I. 23860) mostrou-se eficaz na classifica??o qualitativa e quantitativa do ciclo. Observou-se diferen?as no padr?o citol?gico de animais submetidos aos f?rmacos em estudo; onde o tamoxifeno apresentou alargamento das fases de menor matura??o (diestrais),
enquanto que o anastrozol e o exemestano incrementaram a dura??o das fases de maior matura??o (estrais). O tratamento dos dados atrav?s de uma regress?o n?o linear por spline c?bica permitiu melhor caracteriza??o dos f?rmacos,
sugerindo um perfil citol?gico pr?prio ao antagonismo do receptor de estr?geno (tamoxifeno), competi??o da aromatase (anastrozol) e inibi??o da enzima (exemestano)
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13511 |
| Date | 18 August 2006 |
| Creators | Martins, Rand Randall |
| Contributors | CPF:47015578042, http://lattes.cnpq.br/4311626091295357, Lima, Carlos Eduardo Queiroz, CPF:29368499420, http://lattes.cnpq.br/7570443241449901, Freire, Ana Cl?udia Galv?o, CPF:79221521400, http://lattes.cnpq.br/3215086103105355, Lemos, Telma Maria Araujo Moura |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias Farmac?uticas, UFRN, BR, Bioan?lises e Medicamentos |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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