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The Influence of Various Energy Sources on Microbial Protein Synthesis From Biuret as Determined by Artificial Rumen Fermentation

Barley, cornstarch, crested wheatgrass, glucose, molasses and solka floc were fermented with rumen fluid obtained from sheep that were adapted or unadapted to feed grade biuret (Kedlor) in their diet. Each rumen fluid and substrate combination was subjected to three nitrogen treatments: (1) control, (2) nitrogen added as feed grade biuret and (3) reagent grade biuret in a factoral arrangement of treatments. In vitro fermentations were terminated at 0, 12, 24, and 36 hours and the protein nitrogen insoluble in trichloroacetic acid (TCA) was determined.
Molasses and cornstarch fermentation residues increased while barley, crested wheatgrass, glucose and solka floc decreased in TCA insoluble protein nitrogen. The average of all treatments decreased in TCA insoluble protein nitrogen during the first 12 hours and increased during the remaining 24 hours. Unadapted rumen fluid with simple carbohydrates from glucose and molasses and adapted rumen fluid with complex carbohydrates from barley, crested wheatgrass, cornstarch and solka floc gave the largest gains in TCA insoluble protein nitrogen. No significant differences occurred due to the addition of non-protein nitrogen.

Identiferoai:union.ndltd.org:UTAHS/oai:digitalcommons.usu.edu:etd-6126
Date01 May 1971
CreatorsEggleston, Jenny
PublisherDigitalCommons@USU
Source SetsUtah State University
Detected LanguageEnglish
Typetext
Formatapplication/pdf
SourceAll Graduate Theses and Dissertations
RightsCopyright for this work is held by the author. Transmission or reproduction of materials protected by copyright beyond that allowed by fair use requires the written permission of the copyright owners. Works not in the public domain cannot be commercially exploited without permission of the copyright owner. Responsibility for any use rests exclusively with the user. For more information contact Andrew Wesolek (andrew.wesolek@usu.edu).

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