Grouper in Taiwan is of high value, but nervous necrosis virus infection causes
100% mortality. Our laboratory had developed a good expression system to produce
virus-like particles that induced immune functions. In this study, cells producing
monoclonal antibody against the virus-like particles were used to induce BALB/c mice production of high titer ascites. The ascite from the H1 cells generated 6000-fold of antibodies higher than cell culture, using enzyme-linked immune-sorbent assay, although relatively less than the previous monoclonal cells of mX, 8000-fold. In the electron microscopy, the ascite antibody bound to various mutants of virus-like particles. Using the polymerase chain reaction to amplify fragments of IgG cDNAs, we will clone and express such cDNAs to efficiently produce the desired monoclonal antibodies.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0909111-114035 |
| Date | 09 September 2011 |
| Creators | Liu, Yu-Ting |
| Contributors | Shu-Fen Chiou, Chan-Shing Lin, Wen Zhi-Hong, Chi-Hsin Hsu, Tian-Lu Cheng |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0909111-114035 |
| Rights | user_define, Copyright information available at source archive |
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