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Effects of Nutrient Media on Growth and Morphology of Azotobacter Vinelandii

The work described in this thesis was undertaken to study the reasons why Azotobacter vinelandii ATCC 12837 after incubation in Burk's nitrogen-free liquid will not form as many colonies when plated on Difco Tryptic Soy Agar as when planted on Burk's nitrogen-free agar. The difference in growth of A. vinelandii on the two agars was established by performing viable cell-plate counts. The difference in growth was most apparent at 24-hours incubation of the Burk's liquid-media cultures. Phase contrast microscopic observations of Tryptic Soy media cultures of A. vinelandii disclosed the regular formation of fungoid cells at early stages of growth of the bacteria, 18 to 24 hours.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc935759
Date08 1900
CreatorsButsch, Robert W.
ContributorsVela, G. Roland, 1927-, Norton, S. J.
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatv, 42 leaves : ill., Text
RightsPublic, Butsch, Robert W., Copyright, Copyright is held by the author, unless otherwise noted. All rights

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