Interleukin 17F (IL17F) has been found to be involved in various inflammatory pathologies
and has recently become a target for therapeutic purposes. In contrast to IL17F secreted by immune
cells, the focus of this study is to describe the triggers of IL17F release in non-immune cells with a
particular focus on IL17F-induced fibrosis. IL17F induction was examined in human lung epithelial
(BEAS-2B) and myeloid cell lines as well as in peripheral blood mononuclear cells after in vitro
exposure to aqueous cigarette smoke extract (CSE), inorganic mercury, cadmium or the apoptosis
inducer brefeldin A. Fibrosis was examined in vitro, evaluating the transition of human primary
dermal fibroblasts to myofibroblasts. We observed that all stressors were able to induce IL17F gene
expression regardless of cell type. Interestingly, its induction was associated with cytotoxic/apoptotic
signs. Inhibiting oxidative stress by N-acetylcysteine abrogated CSE-induced cytotoxic and IL17F-
inducing effects. The induction of IL17F was accompanied by IL17F protein expression. The transition
of fibroblasts into myofibroblasts was not influenced by either recombinant IL17F or supernatants of
CSE-exposed BEAS-2B. In addition to IL17F secretion by specialized or activated immune cells, we
underscored the cell type-independent induction of IL17F by mechanisms of inhibitable oxidative
stress-induced cytotoxicity. However, IL17F was not involved in dermal fibrosis under the conditions used in this study.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:90360 |
| Date | 07 March 2024 |
| Creators | Bauer, Mario, Fink, Beate, Anderegg, Ulf, Röder, Stefan, Zenclussen, Ana Claudia |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2073-4425, 10.3390/genes13101739 |
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