Obesity is a metabolic disorder that results in elevated levels of free fatty acids
and triglycerides in the blood circulation, which further leads to accumulation of
lipids within various tissues. Like in other similar metabolic disorders, obesity is
thought to be originated from structural and regulatory changes in
macromolecular assemblies. This current study aims to investigate the effects of
obesity on macromolecular alterations in order to characterize Berlin fat mouse
inbred (BFMI) lines which arenew models for the obesity research that may
contribute to understanding of spontaneous obesity without induction of a high
fat diet. Attenuated Total Reflectance - Fourier Transform Infrared (ATR-FTIR)
spectroscopy was used to characterize content and structure of macromolecules
in male and female control (DBA/2J) and BFMI lines / namely BFMI856,
BFMI860 and BFMI861, in two different adipose tissues / inguinal fat (IF) which
is subcutaneous adipose tissue (SAT), gonadal fat (GF) which is visceral adipose
tissue (VAT). Structural and compositional alterations in proteins, lipids,
saturated and unsaturated lipid contents, nucleic acid, collagen and glycogen
contents and variations in the lipid chain length were determined. BFMI860 and
BFMI861 lines were found to be the most affected lines since they showed the
indications of lipid peroxidation and insulin resistance more severely as they had
lower glycogen content in all tissues and lower unsaturated lipid content
especially in IF adipose tissues. Moreover, structural changes in lipids were
observed especially in male GF adipose tissues of BFMI856 and BFMI861 lines.
Protein content decreased significantly specifically in female IF adipose tissues
but no change was observed in the structure. Furthermore, BFMI852 line was
found to be affected different than other lines and had an effect on especially
female IF. To conclude, obesity induced changes differ in BFMI lines according
to the gender, adipose tissue type and distinctness in the strains.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12614562/index.pdf |
| Date | 01 August 2012 |
| Creators | Sen, Ilke |
| Contributors | Severcan, Feride |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | Access forbidden for 1 year |
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