Cross Reacting Material 197(CRM197) is a Diphteria toxin non toxic mutant that had shown anti-tumor activity in mice and humans. CRM197 is utilized as a specific inhibitor of heparin-binding epidermal growth factor (HB-EGF), that competes for the epidermal growth factor receptor (EGFR), overexpressed in colorectal cancer and implicated in its progression.
We evaluated the effects of CRM197 on HT-29 human colon cancer cell line behaviour and, for CRM197 recognized ability to inhibit HB-EGF, its possible effects on EGFR activation.
In particular, while HT-29 does not show any reduction of viability after CRM197 treatment, or changes in cell cycle distribution, in EGFR localization or activation, they show a change in gene expression profile analyzed by microarray. This is the first study where the CRM197 treatment on HT-29 show the alteration of a specific and selected number of genes.
Identifer | oai:union.ndltd.org:unibo.it/oai:amsdottorato.cib.unibo.it:4730 |
Date | 15 May 2012 |
Creators | Rivetti, Stefano <1983> |
Contributors | Solmi, Rossella |
Publisher | Alma Mater Studiorum - Università di Bologna |
Source Sets | Università di Bologna |
Language | Italian |
Detected Language | English |
Type | Doctoral Thesis, PeerReviewed |
Format | application/pdf |
Rights | info:eu-repo/semantics/openAccess |
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