Regulators of G-protein coupled signaling (RGS) accelerate GTP hydrolysis and consequently influence signal termination. The RGS-4 gene has recently been reported to be implicated in a wide range of neuropsychiatric disorders including schizophrenia, Alzheimer's disease and addictions. / In this study, the vervet RGS-4 gene was sequenced on a CEQ 8000 genetic analysis system (Beckman Coulter) and characterized using molecular and bioinformatic tools. The obtained vervet sequence overall showed 95.3% sequence identity with the human RGS4 gene. / Thereafter, SNPs in the region encompassing the proximal promoter, exon 1 and the first 450 bp of intron 1 were identified by direct sequencing of 8 unrelated individuals. One of the identified SNPs, +35 [A/G], was genotyped in 155 juvenile vervets previously phenotyped for personality traits, including social isolation. Although preliminary association analysis fails to attain statistical significance (p=0.074), the sample size is small. Additional genotyping of phenotypically defined individuals needs to be undertaken.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.84101 |
| Date | January 2004 |
| Creators | Trakadis, John |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Human Genetics.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002573145, proquestno: AAIMR28651, Theses scanned by UMI/ProQuest. |
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