Recent studies have demonstrated that organotypic raft cultures serve as an excellent model for the optical behavior of actual tissues. Other studies in our lab have demonstrated that Raman spectroscopy can discriminate among basal cell carcinoma, squamous cell carcinoma (SCC), non-normal benign, and normal skin in vivo. The primary purpose of this work is to understand the biologic basis of skin Raman spectra and to determine the impact of the location of SCC cells within raft cultures on the spectral bands. Rafts were constructed with SCC cells in the stroma or in the epidermis, and measurements were taken at multiple time points with a Raman probe system. The data allowed tissue discrimination as before, and they showed that the location and concentration of SCC cells do not have a large influence on macroscopically-gathered data. In addition, data gathered from the epidermal vs. stromal layers, combined with histology, support the hypothesis that Raman spectroscopy can detect biochemical changes associated with malignancy before such changes are evident via histology.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-04032006-122308 |
| Date | 17 April 2006 |
| Creators | Keller, Matthew David |
| Contributors | Dr. Anita Mahadevan-Jansen, Dr. Frederick R. Haselton |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-04032006-122308/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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