Previous studies have reported that fluorescence in human tissue is a temperature dependent phenomenon. The most apparent effect is the inverse relationship between fluorescence intensity and temperature. Here, we present the effects of temperature on fluorescence in human tissue using skin and adipose tissue in vitro and skin in vivo. Fluorescence and diffuse reflectance measurements were made while the temperature of the specimens was increased, and an inverse Monte Carlo algorithm was used to calculate optical properties as a function of temperature. In vitro and in vivo experiments showed a decrease in fluorescence intensity due to temperature increase. The fluorescence intensity showed no relationship to the optical properties in the physiological temperature range, suggesting that changes in optical properties are not the primary mechanism by which fluorescence is affected by temperature. This study confirms that fluorescence decreases with increasing temperature in human tissue in vitro and in vivo. Our results further suggest the presence of a temperature dependent non-radiative decay mechanism.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-03262010-093034 |
| Date | 09 April 2010 |
| Creators | Masters, Daniel Barton |
| Contributors | E. Duco Jansen, Anita Mahadevan-Jansen |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-03262010-093034/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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