This thesis relates to the study of rapid ionic currents in single cells using a single electrode voltage clamp amplifier (SEVC). The practical limitations inherent with the SEW, primarily the effects of series resistance (R s) which dominate when attempting to record rapid ionic currents, are outlined. The various methods in use to compensate for Rs are explored. A novel method of Rs compensation is described which overcomes the stability limitations of conventional designs. / To illustrate the advantages of this new Rs compensation, a voltage clamp amplifier implementing this Rs compensation is used to record rapid Na+ current in rat Superior Cervical Ganglia (SCG) neurons and human ventricular myocytes which could not be resolved with conventional equipment. Data obtained using partial Rs compensation is compared to that obtained with full Rs compensation using the new voltage clamp amplifier. Criteria for adequate voltage control of voltage clamped Na+ currents are developed and illustrated.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.21639 |
| Date | January 1998 |
| Creators | Sherman, Adam, 1965- |
| Contributors | Cooper, E. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Physiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001650121, proquestno: MQ50878, Theses scanned by UMI/ProQuest. |
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