The rules and mechanical forces governing cell motility and interactions with the extracellular matrix of a tissue are often critical for understanding the mechanisms by which breast cancer is able to spread through the breast tissue and eventually metastasize. Ex vivo experimentation has demonstrated the the formation of long collagen fibers through collagen gels between the cancerous mammary acini responsible for milk production, providing a fiber scaffolding along which cancer cells can disorganize. We present a minimal mechanical model that serves as a potential explanation for the formation of these collagen fibers and the resultant motion. Our working hypothesis is that cancerous cells induce this fiber formation by pulling on the gel and taking advantage of the specific mechanical properties of collagen. To model this system, we present a hybrid method where we employ a new Eulerian, fixed grid simulation known as the Reference Map Method to model the collagen as a nonlinear viscoelastic material coupled with a multi-agent model to describe individual cancer cells. We find that these phenomena can be explained two simple ideas: cells pull collagen radially inwards and move towards the tension gradient of the collagen gel, while being exposed to standard adhesive and collision forces. From a computational perspective, we hope that our work can serve as a generalizable framework for future theoretical studies of the mechanical interactions between a large number of cells and a dynamic environment.
| Identifer | oai:union.ndltd.org:harvard.edu/oai:dash.harvard.edu:1/14398530 |
| Date | 09 April 2015 |
| Creators | Wang, Jeffrey Bond |
| Publisher | Harvard University |
| Source Sets | Harvard University |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation, text |
| Format | application/pdf |
| Rights | open |
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