The members of the genus Bacillus produce a wide variety of secondary metabolites with
antimetabolic and pharmacological activities. These metabolites are mostly small peptides and have
unusual components and chemical bonds. These metabolites are synthesized nonribosomally by
multifunctional enzyme complexes called peptide synthetases. One of those small peptides,
bacilysin, is a dipeptide antibiotic composed of L-alanine and L-anticapsin which is produced and
excreted by certain strains of Bacillus subtilis. Proteins that are responsible to synthesize bacilysin
are encoded by bac operon. It has been shown that the biosynthesis of bacilysin is under the control
of quorum sensing global regulatory pathway through the action of ComQ/ComX, PhrC (CSF),
ComP/ComA in a Spo0K (Opp)-dependent manner. The objective of the study is to identify the
functional roles of bacilysin biosynthesis in the regulatory cascade and idiophase cell physiology
operating in B. subtilis by using gel-based and gel-free proteomics techniques. For this, we employed
comparative proteome-wide analysis of the bacilysin producer B. subtilis PY79 and its bacilysin nonproducer
derivative bacA::lacz::erm OGU1 strain which was recently constructed by our group.
Identification via GeLC analysis of 76 differentially expressed proteins from total soluble proteome of
wild-type PY79 and bacilysin minus OGU1 strain indicated the direct or indirect multiple effects of
bacilysin on metabolic pathways, global regulatory systems and sporulation.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12615570/index.pdf |
| Date | 01 January 2013 |
| Creators | Demir, Mustafa |
| Contributors | Ozcengiz, Gulay |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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