Theoretical part of the master thesis was aimed on giving an overview of basic characteristics of magnetic particles, their morphology, basic methods of their synthesis, interaction with DNA and recent applications in biotechnology and biomedicine. The experimental part of thesis was aimed on application of new designed magnetic particles for isolation of both lactobacilli DNA and calf thymus DNA. Two types of magnetic beads were used: hyperbranched poly(glycidyl methacrylate-co-[2-(methacryloyloxy) ethoxy]acetic acid-co-ethylene dimethylacrylate) microbeads covered with amino groups (P(GMA-MOEAA-EDMA)-NH2) and magnetic non-porous poly(2-hydroxyethyl methacrylate) microbeads covered with carboxyl groups (P(HEMA-co-GMA)-COOH). For both types of microbeads two different protocols for preparation of separation mixtures with two different concentrations od poly(ethyleneglycol) 6000 (PEG 6000) as condensation agent were tested. Differences among both types of magnetic microbeads and DNAs used were found. It was shown that both types of microbeads are suitable for DNA isolation in the presence of 8% PEG 6000.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:376843 |
| Date | January 2018 |
| Creators | Machan, Radoslav |
| Contributors | Španová, Alena, Rittich, Bohuslav |
| Publisher | Vysoké učení technické v Brně. Fakulta chemická |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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