Bacteriophages affect bacterial evolution, pathogenesis and global nutrient cycling. They are also the most numerous and diverse group of biological entities on the planet [1, 2, 3, 4, 5, 6]. Members of the Lambda phage family share a similar genetic organization and control early gene expression by suppressing transcription, a process known as antitermination. Transcription antitermination in Lambda is mediated by a phage-encoded protein whereas in lambdoid phage HK022, antitermination is mediated by a phage-encoded RNA molecules. Recent results suggest that another bacteriophage called HK639 also appears to use RNA-mediated antitermination. To characterize this newly identified phage we generated site directed mutations and identified where the phage integrates into the chromosome of its bacterial host.
Identifer | oai:union.ndltd.org:WKU/oai:digitalcommons.wku.edu:theses-1041 |
Date | 01 December 2008 |
Creators | Jonnalagadda, Madhuri |
Publisher | TopSCHOLAR® |
Source Sets | Western Kentucky University Theses |
Detected Language | English |
Type | text |
Format | application/pdf |
Source | Masters Theses & Specialist Projects |
Page generated in 0.0015 seconds