We explain a new method of detecting non point source fecal contamination
using a PCR based method called Touchdown Polymerase Chain Reaction (TD-PCR).
Using genetic markers particular to general, ruminant and human Bacteroides-
Prevotella genes, we identified presence in both fresh and salt water environments.
Water samples from four sites were collected at approximate 2-week intervals for a
year. Samples were analyzed for total coliforms, fecal coliforms, E. coli, and the
presence of general, ruminant and human Bacteroides-Prevotella markers. We
compared the odds of recovering each PCR marker between sites. We investigated the
relationship between rainfall and recovery of PCR markers. Finally, we compared the
sensitivity of the PCR methods to standard public health methods. / Graduation date: 2003
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/31527 |
| Date | 12 February 2003 |
| Creators | Jones, Thomas E. |
| Contributors | Rossignol, Annette M. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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