This study was conducted to assess the incidence of infectious drug resistance among animal strains of Escherlchia coli. Fecal samples were examined from a cattle feedlot southeast of Muncie, Indiana. The use of tetracycline and sulfonamide compounds as feed additives was a common practice on this farm, and theoretically could provide the antibiotic pressure necessary to select for resistant strains. A total of 32 feedlot E. coli were isolated from the feedlot floor. In addition, 10 strains of E. coli isolated from patients with urinary tract infections were introduced into this investigation for comparative purposes. All isolates were tested for antibiotic susceptibility using disc-diffusion methods. Multiple resistant strains were designated as donor organisms and were used in conjugation experiments with an antibiotic sensitive recipient E. coli; wild type K-12 F-.Multiple drug resistance occurred among 21.8 percent of the feedlot E. coli isolated, with a predominance of resistance to chlorotetracycline, tetracycline, and sulfathiazole. Three out of seven multiple resistant donors isolated were able to transfer their antibiotic resistance markers to the recipient K-12 strain. Transferable drug resistance among the clinical isolates occurred in 6 of the 10 donor resistant strains, with tetracycline and ampicillin being the most frequently encountered resistance.Ball State UniversityMuncie, IN 47306
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/180831 |
| Date | 03 June 2011 |
| Creators | Porter, Timothy E. |
| Contributors | Hendrickson, Donald A. |
| Source Sets | Ball State University |
| Detected Language | English |
| Format | vi, 41 leaves : ill. ; 28 cm. |
| Source | Virtual Press |
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