A modification of bulked segregant analysis was used to raise the density of markers in a 34.5 cM region between Ugp2 and Ugp1, on chromosome 3 of the Harrington x TR306 barley Hordeum vulgare L.) cross. A computer program was used to select pools contrasting for parental alleles at the target site. Of 257 RAPD primers tested on DNA pools, one, UBC 508, detected a polymorphic DNA fragment (UBC508(C)). It mapped 10.2 cM distal to Ugp2. Two additional DNA polymorphisms, (UBC508(A) and UBC508(B)), mapped on chromosome 2. An additional marker, BCD 1796B, mapped 4.9 cM proximal to Ugp1. Both strands of the UBC508(C) fragment were sequenced. They were 588 bp long and had some homology to a region of the DNA that regulates transcription of the H. vulgare pazx gene encoding protein Zx. The effect of adding new marker(s) on the QTL analysis of agronomic and quality traits of barley, was investigated. For extract beta-glucan, a new peak was identified in the analysis when only UBC508(C) or when both UBC508(C) and BCD1796B were added. For fine-coarse difference a QTL x E interaction peak was detected when only BCD 1796B was added or when both UBC508(C) and BCD 1796B were added.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.20809 |
| Date | January 1998 |
| Creators | Campeol, Nadia. |
| Contributors | Fortin, M. G. (advisor), Mather, D. E. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Plant Science.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001642306, proquestno: MQ44137, Theses scanned by UMI/ProQuest. |
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