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Previous issue date: 2014-09-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Ganoderma lucidum (Curtis) P. Karst is a medicinal fungus (Basidiomycota, Polyporaceae) popularly known as "Lingzhi" in China, "Reishi" in Japan and "Mushroom King" in Brazil, which has been cultivated in several lignocellulosic materials. In the present study, the formation of mycelium as well as the production of ligninolytic enzymes and basidiomata of this species were evaluated in waste fruit shell, leaf and bract endemic palm of the Brazilian semi-arid region, Syagrus coronata (Martius) Beccari (licuri) by solid state fermentation. The optimal cultivation conditions were the same for all three types of substrates tested (pH 6.5, C/N ratio of 40 and 30?C of temperature) and were established from assays in Petri dishes and contour graphic analysis and response surface methodology. The enzymatic activity of laccase and manganese peroxidase were determined using spectrophotometric methods at intervals of 7 days by cultivating the strain in the three substrates selected for 28 days of incubation. The activity of the laccase was determined at 420 nm, using ABTS: 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) as the enzymatic substrate, and the activity of the manganese peroxidase at 610 nm by oxidation of phenol red. The highest peak activity for laccase (13,80 U/L) was found in bract substrate in 14 days of incubation and did not differ significantly from the others at 95% of statistical confidence. That substrate also resulted in the highest peak of activity for manganese peroxidase (14,92 U/L), which only occurred after 28 days of incubation, differing from the previous one at 5% probability. The assay for the production of basidiomata was performed in polypropylene bags. The only substrate that did not promote the formation of the basidiomata was the fruit shell. The biological yield did not differ significantly at 95% confidence in the bract (33,53 g/kg) and leaf (37,48 g/kg) substrates. The biological efficiency also was not statistically different in the bract (3,35%) and leaf (3,75%) substrates. Thus, it is believed that the licuri wastes are potential substrates and can be used in the bioconversion processes for the formation of the mycelium and basidiomata as well for ligninolytic enzymes production in G. lucidum. / Ganoderma lucidum (Curtis) P. Karst ? um fungo medicinal, do Filo Basidiomycota, Fam?lia Polyporaceae, popularmente conhecido como ?Lingzhi? na China, ?Reishi? no Jap?o e ?Cogumelo Rei? no Brasil, que tem sido cultivado em diversos materiais lignocelul?sicos. No presente estudo, foram avaliadas a forma??o de mic?lio, bem como a produ??o de basidiomas e enzimas ligninol?ticas da esp?cie em res?duos de casca de fruto, folha e br?ctea da palmeira end?mica da regi?o semi-?rida brasileira, Syagrus coronata (Martius) Beccari (licuri), por meio de fermenta??o em estado s?lido. As condi??es ?timas de cultivo foram as mesmas para os tr?s tipos de substratos testados (pH 6,5, rela??o C/N de 40 e temperatura de 30?C), sendo estabelecidas a partir do ensaio em placas de Petri e an?lise dos gr?ficos de contorno e superf?cie de resposta. A atividade enzim?tica de lacase e de mangan?s peroxidase foram determinadas empregando m?todos espectrofotom?tricos, em intervalos de 7 dias, atrav?s do cultivo da linhagem nos tr?s substratos selecionados durante 28 dias de incuba??o. A atividade de lacase foi determinada a 420 nm, empregando ABTS: 2,2?-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) como substrato enzim?tico e a de mangan?s peroxidase, a 610 nm, pela oxida??o do vermelho de fenol. O maior pico de atividade para a lacase (13,80 U/L) foi verificado no substrato br?ctea, aos 14 dias de incuba??o, e n?o diferiu significativamente dos demais a 95% de confian?a. Esse substrato tamb?m proporcionou o maior pico de atividade para a mangan?s peroxidase (14,92 U/L), que somente ocorreu aos 28 dias de incuba??o, diferindo estatisticamente dos demais a 5% de probabilidade. O ensaio para a produ??o de basidiomas foi realizado em sacos de polipropileno. O ?nico substrato que n?o promoveu a forma??o de basidiomas foi a casca de fruto de licuri. O rendimento biol?gico n?o diferiu significativamente a 95% de confian?a nos substratos br?ctea (33,53 g/kg) e folha (37,48 g/kg). A efici?ncia biol?gica tamb?m n?o foi estatisticamente diferente nos substratos br?ctea (3,35%) e folha (3,75%). Assim, acredita-se que os res?duos de licuri sejam potenciais substratos e possam ser empregados no processo de bioconvers?o para a forma??o de mic?lio e basidiomas, e tamb?m para a produ??o de enzimas ligninol?ticas de G. lucidum.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.uefs.br:8080:tede/514 |
| Date | 30 September 2014 |
| Creators | Menezes, Tha?s Almeida de |
| Contributors | G?es Neto, Arist?teles |
| Publisher | Universidade Estadual de Feira de Santana, Mestrado Acad?mico em Biotecnologia, UEFS, Brasil, DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UEFS, instname:Universidade Estadual de Feira de Santana, instacron:UEFS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -5473543273051652782, 600, 600, 600, 600, 600, 5026123383450589282, -3439178843068202161, 1258845414555579790, 3590462550136975366 |
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