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Production of conjugated linoleic acid and conjugated linolenic acid by Bifidobacterium breve JKL03 and its application

Conjugated linoleic acid (CLA) is predominantly found in foods of ruminant origin such as milk and processed cheese, and has gained much interest recently due to its beneficial health and biological effects on animals and humans. / The bioconversion of linoleic acid (LA) and linolenic acid (LNA) by a selected Bifidobacterium from healthy infant feces was studied. Bifidobacterium breve JKL03 had the ability to convert linolenic acid (0.2 mg/ml) to CLNA in fermentation of skim milk medium for 24 h up to a yield of 72.0% (up to 74.7% under aerobic conditions) and linoleic acid (0.2 mg/ml) into CLA by fermentation in skim milk medium for 24 h up to a yield of 23.9% (up to 28.0% under aerobic conditions). / B. breve JKL03 was also co-fermented with Lactobacillus acidophilus (NCFMRTM strain), a commonly added starter culture, to observe the resulting effects on growth during fermentation for yogurt production. Fermentation of LNA in skim milk with B. breve JKL03 and L. acidophilus (NCFM) maintained high CLNA production level. On the other hand, CLA production in the same media with both strains did not exhibit as high level as with the single B. breve. / These results are important for the advancement of knowledge on the production of CLA and CLNA in dairy products and for knowledge on the basic metabolic mechanisms for such conversion.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.82258
Date January 2005
CreatorsJung, Yun-Kyoung, 1979-
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageMaster of Science (Department of Food Science and Agricultural Chemistry.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 002227061, proquestno: AAIMR12469, Theses scanned by UMI/ProQuest.

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