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Charakterizace a význam N-glukosyltransferázové dráhy jako nástroje regulace homeostáze cytokininů v rostlinách / Characterization and significance of N-glucosyltransferase pathway as a tool for regulation of cytokinin homeostasis in plants

Adenine derivatives called cytokinins (CK) are a group of plant hormones, which in cooperation with other plant hormones orchestrates almost every aspect of plant growth and development. Despite the rapid progress in plant hormone research, there are still many aspects we may shed light on due to the metabolic and signalling pathways redundancy and the network complexity with crosstalk hubs. CK N-glucosides (in this case trans-zeatin-7-glucoside, tZ7G, and trans-zeatin-9-glucoside, tZ9G) have been traditionally viewed as irreversibly deactivated products of CK active form metabolism (in this regard trans-zeatin, tZ). Nevertheless, the tZ9G antisenescent activity was shown in oat leaf senescence bioassays (Avena sativa cv. Abel) and the possibility of metabolic conversion to O-glucosides was hypothesized. The aim of this work was to test the hypothesis on the close model of oat (Avena sativa cv. Patrik) and to examine also the metabolic conversion of related substances - N 6 -(Δ2 -isopentenyl)adenine (iP) and its N7- and N9- glucosides (iP7G and iP9G). While the senescence retardation caused by exogenous tZ9G application was confirmed in the Avena sativa cv. Patrik, metabolic conversions to O-glucosides remain to be verified. Besides the effects of above-mentioned substances on the oat leaf...

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:403990
Date January 2019
CreatorsDoležálková, Lucie
ContributorsMotyka, Václav, Vaňková, Radomíra
Source SetsCzech ETDs
LanguageEnglish
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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