Murine decidual cells were examined for surface markers common to lymphomyeloid cells and for unique lineage specific markers by radioautography. They were shown to be Thy-1('+), Mac-1('(+OR-)), I-A('-), I-J('-) and Lyt('-). Two protein-A binding, IgG2b isotype monoclonal antibodies recognizing unique decidual cell surface antigen(s) were produced by immunizing virgin CBA mice with syngeneic decidual cells. Using radioautography and immunofluorescence, the incidence of decidual cells expressing these antigen(s) was found to rise with advancing gestation. They were also present on decidual cells of other mouse strains, rats, and humans, but not detectable on lymphomyeloid cells in any of these species. / Leukocyte subsets infiltrating the murine decidua basalis were also characterized and quantitated. A biphasic rise in total cellularity, more pronounced during allogeneic than syngeneic pregnancy, was attributable primarily to decidual cells and to a smaller extent lymphocytes, amongst which null (IgM('-), Thy-1('-)) cells predominated. Lyt-1 cells were the most frequent T cell subset, however, a terminal rise in the Lyt-2 subpopulation occurred in allogeneic pregnancy. Only a subpopulation of macrophages expressed I-A and all expressed Mac-1 antigens. / These findings provide a basis for further functional studies of the decidua.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.71978 |
| Date | January 1984 |
| Creators | Kearns, Mary, 1957- |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Anatomy.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000213530, proquestno: AAINL20839, Theses scanned by UMI/ProQuest. |
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