Epididymides post-fixed in reduced osmium were used to study the morphological characteristics of the Golgi apparatus of principal cells of the initial segment. Others, fixed in glutaraldehyde, were treated to detect nicotinamide adenine dinucleotide phosphatase (NADPase), thiamine pyro-phosphatase (TPPase), and cytidine mono-phosphatase (CMPase) activities within Golgi compartments and glucose-6-phosphatase activity localized in cisternae of ER. / The Golgi apparatus of these cells is composed of numerous saccular and tubular elements. On the cis face of the Golgi there is an osmiophilic tubular network. Of the numerous subjacent saccules, the first is dilated and unreactive for phosphatases, the next 3 show NADPase activity and the last 4 are TPPase positive. On the trans aspect of the stack there are 3 or 4 CMPase positive trans Golgi networks, one showing a peeling off configuration while the other 2 or 3 are completely separated from the stack. The TGN's often contain nodular distensions suggestive of secretion granule formation and occasionally present a shrivelled appearance. / Cisternae of ER showing buds are present on the cis face of the stacks.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.59246 |
| Date | January 1990 |
| Creators | Green, Harold G. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Anatomy.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001233017, proquestno: AAIMM63427, Theses scanned by UMI/ProQuest. |
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