Mouse L cell mutants defective in receptor mediated endocytosis were isolated using selection with modeccin followed by screening for loss of mannose 6-phosphate (Man 6-P) dependent uptake or for resistance to Pseudomonas exotoxin. Mutants L146 and L15D were resistant to modeccin, lacked 215 kD Man 6-P receptor, were defective in Man 6-P dependent uptake and binding, and oversecreted several lysosomal enzymes. Mutant LEFIC was resistant to modeccin, Pseudomonas exotoxin and ricin. The mutant exhibited delayed (1) oligosaccharide processing of VSV G protein, (2) transport of VSV G to the cell surface and (3) release of radiolabeled VSV G into virions. LEFIC was blocked in proteolytic processing of the Sindbis glycoprotein pE2 and showed intracellular accumulation of Sindbis nucleocapsid. The mutant showed a delay in secretion of fibronectin. An intersection of the secretory and endocytic pathways is suggested by the isolation of this cross-toxin resistant mutant defective in intracellular transport of membrane and secretory proteins.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.75901 |
| Date | January 1988 |
| Creators | Laurie, Susan M. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Anatomy.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000910426, proquestno: AAINL52249, Theses scanned by UMI/ProQuest. |
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