The main objective of this study was to determine the distribution of these perforatorial polypeptides in the head of the rat spermatozoon. / Immunoblotting demonstrated that seven perforatorial polypeptides tested in this study, had epitopes in common. Immunogold labeling of spermatozoa showed that the distribution of the 34, 43, 57 and 63 kDa polypeptides corresponded exactly to the regions of the perinuclear theca immunolabeled with the whole perforatorium serum. However, antibodies affinity purified against the 13, 13.4 and 16 kDa polypeptides were restricted in their localization to the thicker apical portion of the perforatorium and to the inner layer of the ventral spur. / These results suggest (1) the perforatorium is biochemically distinct from the postacrosomal sheath with the exception of a restricted region referred to as the ventral spur; (2) there are regional differences in protein composition of the perforatorium, of the outer periacrosomal layer and of the postacrosomal sheath; and (3) that perforatorial polypeptides may not necessarily be restricted to the subacrosomal region, by may also compose portions of the outer periacrosomal layer, and postacrosomal sheath; and (4) Actin is not part of the perforatorium in mature rat spermatozoa.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.56632 |
| Date | January 1992 |
| Creators | Moussakova, Linda |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Anatomy.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001307898, proquestno: AAIMM80401, Theses scanned by UMI/ProQuest. |
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