The events in the formation of the components of the mammalian sperm flagellum have been elucidated, in an analysis of rat spermiogenesis, by electron microscopy and EM radioautography using ('3)H-proline and ('3)H-cystine. The connecting piece forms by deposition of dense material around the centrioles during steps 8-15. Newly-synthesized proteins are incorporated into this structure throughout this period. The outer dense fibers exist in step 8-14 spermatids as slender fibrils associated with the axonemal doublets. During step 16, they undergo rapid growth, assuming the form of the mature fibers. This results from massive deposition of proline- and cystine-containing proteins onto the fibrils. The fibrous sheath develops from a framework comprised of two longitudinal columns and a series of circumferentially-oriented ribs which is assembled during steps 11-15 in a distal-to-proximal direction along the flagellum. New ribs originate from proline-rich fuzz-coated filaments which arise within the flagellar cytoplasm.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68568 |
| Date | January 1980 |
| Creators | Irons, Margaret Jean. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Anatomy) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000092295, proquestno: AAINK51984, Theses scanned by UMI/ProQuest. |
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