The aim of the study was to investigate the mechanisms of bronchoconstriction induced by dry gas hyperpnea challenge in the rat. The interaction of airway hyperresponsiveness to contractile agonists and the atopic constitution of the animals with responsiveness to hyperpnea challenge was also evaluated. A hyperresponsive strain, Fisher 344, two control normoresponsive strains, Lewis and ACI, and an atopic but normoresponsive rat, Brown Norway (BN) to dry gas hyperpnea challenge were studied. The effects of the NK1 (CP-99994), the NK2 (SR-48968) receptor antagonists, and the selective LTD4 receptor antagonist pranlukast on responses to hyperpnea challenge were examined in BN rats. The animals were anesthetized with xylazine and pentobarbital sodium intraperitoneally and mechanically ventilated with a tidal volume of 8 ml/kg and a frequency of 150 breaths/min for hyperpnea challenge. Pulmonary responses to challenge were measured in spontaneously breathing animals. Dry gas hyperpnea challenge was performed with a dry mixture of 5% CO2-95% O2 for 5 min whereas wet gas was humidified prior to administration. Bronchovascular leakage was assessed by quantitating Evans blue in bronchoalveolar lavage (BAL) fluid. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.20607 |
| Date | January 1997 |
| Creators | Yang, XiaoXia. |
| Contributors | Martin, J. G. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Physiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001604634, proquestno: MQ44318, Theses scanned by UMI/ProQuest. |
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