This work has focused on understanding in vivo aspects of ASP metabolism particularly postprandially. I showed that ASP was produced directly from human adipose tissue, a process that increased postprandially and was correlated with increased plasma triglyceride clearance and increased fatty acid incorporation into adipose tissue "FIAT". The target of ASP is adipose tissue. Binding studies in freshly obtained adipose tissue demonstrated specific binding to high affinity receptor sites. ASP binding to subcutaneous tissue from obese females demonstrated the greatest binding and highest receptor affinity. In contrast omental tissue particularly from males showed the lowest specific binding and affinity. This suggests an important role for ASP in maintaining regional adipose tissue mass in females and males, hence providing possible explanations for the metabolic complications seen in abdominal obesity. / The strongest evidence for a physiological role of ASP on triglyceride clearance for ASP in vivo was obtained when exogenous intraperitonial hASP was administered to genetically obese mice. Normolipidemic ob /ob mice demonstrated accelerated postprandial TG clearance in the presence of ASP. The effect in the hyperlipidemic: db/db mice, however was markedly greater (2 to 8 times). These findings strongly support the hypothesis that ASP is an important factor in postprandial lipid metabolism and may be a significant factor in determining the pathophysiology of obesity and related dyslipidemias.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.35938 |
| Date | January 1998 |
| Creators | Saleh, Jumana. |
| Contributors | Cianflone, K. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001650803, proquestno: NQ50253, Theses scanned by UMI/ProQuest. |
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