<p> The redistribution of nitrogen from old leaves to young leaves during senescence is crucial for plant survival. The <i>Arabidopsis thaliana </i> chloroplast residential protease S33Ab (At5g11650) was predicted to contribute to the degradation of chloroplast stromal proteins during senescence by bioinformatics tools. Rubisco degradation did not co-segregate with the T-DNA insertion mutant <i>s33Ab.</i> The location of T-DNA insertions into <i>S33Ac</i> (At1g18360) and <i>S33Ad</i> (At1g73480) were confirmed by sequencing. Total leaf proteins from <i>Ab, Ac, Ad </i> single, <i>Ab/Ac, Ab/Ad, Ac/Ad</i> double and <i> Ab/Ac/Ad</i> triple mutants were isolated from senescing leaves, subjected to immunoblot analysis to quantify Rubisco large subunit (LSU), glutamine synthase 2 (GS2), Rubisco activase (RCA), and light harvesting complex protein for photosystem II, b (Lhcb1) and compared to wild type. There is no significant difference among all the genotypes. This genetic analysis shows that S33Ab and its two closely related homologs, S33Ac and S33Ad do not individually contribute or work additively in chloroplast stromal protein degradation.</p>
| Identifer | oai:union.ndltd.org:PROQUEST/oai:pqdtoai.proquest.com:1522627 |
| Date | 04 May 2013 |
| Creators | Dou, Xiaoyu |
| Publisher | California State University, Long Beach |
| Source Sets | ProQuest.com |
| Language | English |
| Detected Language | English |
| Type | thesis |
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