All living organisms, when confronted with a number of different environmental changes, including heat shock, elicit a common and seemingly highly conserved response: the heat-shock response. The response is characterized by the rapid, preferential synthesis and accumulation of heat shock proteins (hsps). The multiplicity of responses of Neurospora crassa to a simple thermal stress has been analysed at several levels, molecular and cellular. / The major changes in gene expression occur in mycelia and conidia as their environmental temperature is increased from normal value (25(DEGREES)C) to those elevated temperature (36-48(DEGREES)C). At least eight hsps were greatly synthesized and simultaneously most of preexisting normal proteins were greatly reduced. Each hsp requires the different range of elevated temperatures and different lengths of time for initiation and maximal synthesis. / The response of Neurospora to heat shock was transient during the continous heat-shock treatment: normal protein synthesis resumes and heat shock protein synthesis decreases. / The heat-shock response was differentially expressed during the germination of conidia. Most of hsps were fully expressed only after 3 hours of pre-germination from dormant conidia. / There is a good correlation between the induction of thermotolerance and increased synthesis of hsps. The transient pattern of induction, development, and decay of thermotolerance was very similar to the pattern of hsp synthesis at the same elevated temperature. / The protocol for isolation of several hsps was developed using combination of two different chromatographic methods, including DEAE-HPLC and SE-HPLC. / DNA sequences of Neurospora nuclear genome were cloned, which are highly expressed in heat-shocked cells.(' ) / Source: Dissertation Abstracts International, Volume: 48-03, Section: B, page: 0643. / Thesis (Ph.D.)--The Florida State University, 1987.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_76092 |
| Contributors | LEE, KIL JAE., Florida State University |
| Source Sets | Florida State University |
| Detected Language | English |
| Type | Text |
| Format | 235 p. |
| Rights | On campus use only. |
| Relation | Dissertation Abstracts International |
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