Zinc cluster proteins are a major class of transcriptional regulators in Saccharomyces cerevisiae. This protein family includes Uga3 and Dal81, which are both required for the activation of genes involved in the catabolism of gamma-aminobutyrate (GABA). We discovered that the region of Uga3 encompassing amino acids 130 to 300 likely corresponds to the regulatory domain of the protein. We also determined that both the region encompassing amino acids 300 to 350 and the C-terminus of Uga3 are required for activation. Additionally, activation by Uga3 can occur in strains unable to completely degrade GABA or lacking the GABA permease Uga4. Uga3 can form homodimers in vivo, both in the presence and in the absence of GABA. However, it seems that Da181 cannot form heterodimers with Uga3 nor interact with the UGA1 promoter. Furthermore, Dal81 is no longer required for activation if Uga3 is overexpressed or mutated to a constitutively active form.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.84078 |
| Date | January 2005 |
| Creators | Sylvain, Marc-André |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Microbiology and Immunology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002261581, proquestno: AAIMR22770, Theses scanned by UMI/ProQuest. |
Page generated in 0.0014 seconds