The frequency of transformation of rat cells obtained with recombinant plasmid DNAs which contained an intact early region of the polyoma genome (Bam clones) was not significantly different from those which carry an interrupted early region (Eco RI clones) or even part of the early region (Hind III-1 fragment transformant). The viral DNA within several Bam and Eco RI clones as well as fragment transformants was examined by Southern blotting. These cell lines contain integrated viral sequences which sometimes are tandemly arranged. We also found that the presence of large T antigen in transformed cells does not significantly alter the frequency at which tandem integration occurs. Furthermore, Rat-1 cells in culture were transformed with cellular DNA isolated from one Hind III-1 fragment transformant. These studies showed that the oncogenic potential of the viral DNA is maintained after integration. These findings demonstrate that only part of the polyoma early region is required to initiate and maintain transformation.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68574 |
| Date | January 1980 |
| Creators | Moreau, Pierre |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Microbiology and Immunology) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000092289, proquestno: AAINK52039, Theses scanned by UMI/ProQuest. |
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