Mu is a temperate, transposable coliphage with an unusual mode of replication and maturation. Mu is encapsidated linked to host sequences flanking the dispersed, integrated phage genomes. For encapsidation to occur, phage genomes must be recognized and removed from the chromosome. A putative enzyme recognizes the specific pac sequence within Mu left end. / The objective of my research is to characterize the influence of selected E. coli sequences on Mu transduction. In transduction the virion prohead is filled up with an important percentage, if not in totality, of host DNA. Cryptic pac sequences are defined as E.coli K12 genomic sequences showing the highest homology with the endogenous Mu pac sequence. Those host sequences could influence Mu packaging process towards transductants rather than "standard" infectious phage particles. / Using ClustralW software, two cryptic pac sequences were identified. Transduction assays with cloned cryptic pac sequences give insight into E.coli intrinsic potential to promote transduction.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.81332 |
| Date | January 2004 |
| Creators | Franceschetti, Marie-Laure |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Microbiology and Immunology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002166367, proquestno: AAIMR06395, Theses scanned by UMI/ProQuest. |
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