This project outlines a novel method for testing the survival and inactivation of viral pathogens on viable human tissue carriers. The two topically applied chemical agents selected for testing in the ex vivo model were chlorhexidine digluconate (CD) and benzalkonium chloride (BC). The ex vivo method was developed to use animate carriers: pieces of human skin excised during routine plastic surgery for the stratum corneum and umbilical cords as mucous membranes. With both specimen types, the tissue was mounted on a cutting board and 2.3 cm diameter patches were then made with a cutting tool. Each patch was mounted over the top of a specially designed holder and secured with an O-ring. The animate carriers were then ready for use in the ex vivo protocol. The ex vivo model was evaluated with Human Herpesvirus 2 (HHV 2) strain 333 and Adenovirus 4 (AD 4) strain RI-67 with parallel tests run on the tissues and stainless steel disks to compare virus survival and inactivation on animate and inanimate surfaces. HHV 2 and AD 4 virus pools were made by infecting confluent Vero and 293 cell monolayers, respectively. Throughout the study infectivity for both viruses was measured by plaque assay in Vero cell monolayers. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:uottawa.ca/oai:ruor.uottawa.ca:10393/9633 |
| Date | January 1997 |
| Creators | Graham, Mary Louise. |
| Contributors | Sattar, S. A., |
| Publisher | University of Ottawa (Canada) |
| Source Sets | Université d’Ottawa |
| Detected Language | English |
| Type | Thesis |
| Format | 95 p. |
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