Previous work has shown that some Neisseria meningitidis strains do not express the Class 1 Outer Membrane Protein (OMP1; the porA gene product) as assessed by SDS-PAGE analysis of OMPs. Northern analysis has identified the level of control of porA expression as transcriptional. Promoter regions differed in the number of guanosine residues in a poly(G) track located between the -10 and the -35 regions. Strains that do not express the protein either had an adenosine residue within this poly(G) track or contained nine or less guanosine residues in this track. We investigated the role of the promoter region of porA in the regulation of transcription. The promoter regions of the porA gene from three strains, two of which do not express the OMP1, were analyzed. Promoters were cloned into a promoter-probe vector (pDN19/ acO) possessing a promoterless lacZ gene and expressed in Escherichia coli. Transcription activities of cloned promoter regions were measured by beta-galactosidase assays. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.20975 |
| Date | January 1998 |
| Creators | Sawaya, Rana. |
| Contributors | Mills, Elaine L. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Microbiology and Immunology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001630967, proquestno: MQ50871, Theses scanned by UMI/ProQuest. |
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