Neural tube defect is one of the leading causes of birth defect in human. Mouse planar cell polarity genes Vangl2 and Vangl1 are expressed in the developing neural tube. Mutation in Vangl2 has been shown to cause mouse equivalent of craniorachischisis. A cre/loxP conditional Vangl1 knockout was designed from a pFlox vector. The effectiveness of the cre/loxP recombination in pFlox was tested in a bacterial system. The targeting vector was transfected into pluripotent E.S. cells to allow homologous recombination to displace the wildtype Vangl1. Cre expressing vector was transfected into the E.S. cells to produce both the conditional and conventional Vangl1 knockout.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.99175 |
| Date | January 2005 |
| Creators | Han, Steven. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biochemistry.) |
| Rights | © Steven Han, 2005 |
| Relation | alephsysno: 002330805, proquestno: AAIMR25079, Theses scanned by UMI/ProQuest. |
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