MEIS1 (myeloid ecotropic viral integration site 1) is a member of the TALE class of homeodomain-containing proteins. MEIS1, along with related family members PBX and PREP1, bind DNA cooperatively with HOX proteins to regulate transcription. Meis1 is implicated in many developmental processes. Moreover, deregulation of Meis1 is involved in leukemogenesis. To better understand the mechanisms of Meis1 transcriptional regulation, we set out to map and characterize the Meis1 promoter. Results from RNase protection and 5' RACE suggest that Meis1 has multiple transcription start sites (TSSs) located between -608 and -751 base pairs upstream of the translational start site. Sequence analysis of the promoter region did not reveal a TATA box-like element. However, Meis1 lies within a CpG island, consistent with a clustering of TSSs. Meis1 is induced upon RA treatment of P19 cell aggregates, consistent with H3 and H4 acetylation and RNA pol II recruitment at the Meis1 promoter. Furthermore, MEIS1 is recruited to a conserved MEIS/PBX binding site within the promoter region suggesting that MEIS1 may autoregulate its own transcription.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.83966 |
| Date | January 2005 |
| Creators | Bodner, Caroline |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002261476, proquestno: AAIMR22705, Theses scanned by UMI/ProQuest. |
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