In this work, we characterized the novel mouse orphan receptor, Estrogen-Related Receptor gamma (ERRgamma). ERRgamma is closely related to the other ERR family members. ERRalpha and ERRbeta. ERRgamma binds to both an ERR response element (ERRE) and an estrogen response element (ERE) and displays constitutive transcriptional activity which is enhanced when ERRgamma is fused to the VP16 activation domain. ERRalpha, ERRbeta, and ERRgamma heterodimerize both on an ERE and in solution in absence of DNA. This may allow for a more versatile mode of gene regulation. Furthermore, the ligand-binding domain (LBD) of ERRgamma interacts with coactivators and corepressors in vitro. Expression of ERRgamma in mouse embryogenesis starts at 7.5 until 10.5 day stage and is observed in the ventricular zones of the brain and the spinal cord. This suggests a role for ERRgamma in the developing nervous system. Although ERRgamma is more closely related to ERRbeta than ERRalpha, the expression pattern of ERRgamma is similar to ERRalpha and distinct to ERRbeta, suggesting a unique role for ERRgamma in development.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.31212 |
| Date | January 2001 |
| Creators | Couture, Marie-Claude. |
| Contributors | Giguere, Vincent (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biochemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001802157, proquestno: MQ70404, Theses scanned by UMI/ProQuest. |
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