Chromogranin A (CgA) is an acidic glycoprotein that is specifically expressed in neuroendocrine tissue. It is the best characterized member of the granin family, which includes proteins proposed to be involved in the regulation of peptide hormone and neurotransmitter secretion in the regulated secretors pathway by helping granule formation, targeting peptide hormones and neurotransmitters to the granules, affecting peptide hormone processing and controlling secretion through a feedback mechanism. / Although elaborate in vitro studies have been conducted on CgA, its precise neuroendocrine function and the complete elements that control its neuroendocrine specific expression remain unclear. This project applied transgenic technology to study CgA regulation and function in vivo. For the study of the regulation of CgA gene expression, four constructs, containing one of two promoter portions of the CgA gene (the 184bp proximal promoter portion and the 6kb 5'-flanking region) in two reporter systems (beta-galactosidase and green fluorescent protein systems), were constructed. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.31237 |
| Date | January 2000 |
| Creators | Hou, Yu, 1971- |
| Contributors | Hendy, G. N. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001802386, proquestno: MQ70433, Theses scanned by UMI/ProQuest. |
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