Sequences at both 5$\sp\prime$ and 3$\sp\prime$ ends of mouse histone genes contribute to the expression of the gene. The effect of 5$\sp\prime$ sequences of H2a(614) and H3.2(614) is presumably on the rate of transcription. The 3$\sp\prime$ sequences required for high expression of the mouse H2a(614) gene are the same as the sequences required for 3$\sp\prime$ end formation. When these sequences are substituted for the 3$\sp\prime$ end of the poorly expressed H2a(291) gene, the expression of the H2a(291) gene is increased 5-fold. A 65 nucleotide fragment containing the H2a(614) 3$\sp\prime$ processing signal increases the expression of the H2a(291) gene when it is placed in the proper orientation downstream of the H2a(291) 3$\sp\prime$ end, suggesting that the transcript is sequentially processed. In in vitro processing experiments, the different histone 3$\sp\prime$ ends show different processing efficiencies which correlate with the expression in cells. This hairpin loop structure has multiple uses which include transcription termination, mRNA stability and efficiency of processing by the cell to regulate the steady-state level of histone mRNAs. / Source: Dissertation Abstracts International, Volume: 50-05, Section: B, page: 1785. / Major Professor: William F. Marzluff. / Thesis (Ph.D.)--The Florida State University, 1989.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_78016 |
| Contributors | Liu, Ta-Jen., Florida State University |
| Source Sets | Florida State University |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | 191 p. |
| Rights | On campus use only. |
| Relation | Dissertation Abstracts International |
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