EM radioautography was used to examine the tissue distribution of cells exhibiting nucleoplasmic labeling after being exposed to $ sp3$H-sugars or $ sp{35}$S-sulphate to indicate the general extent of the occurrence of nucleoplasmic glycoproteins within animal cells. The observation of some degree of such labeling in virtually all cells in tissues of three animal species suggests that nucleoplasmic glycoproteins are a common cellular feature. To better define the distribution and nature of the putative labeled nucleoplasmic glycoproteins, cultured cells were used as a model cell type for both quantitative EM radioautographic and biochemical studies. After exposure to $ sp3$H-sugars, all three lines of cultured cells examined exhibited significant nucleoplasmic reaction in which the euchromatin, heterochromatin and nucleoli were all labeled to some extent. Studies on isolated, envelope-depleted nuclei from myeloma cells confirmed that the molecules in the nucleoplasm itself were the source of the radioautographic reaction observed over nuclei. Biochemical analyses of fractions of isolated nuclei indicated that much of the label resided in nuclear matrix glycoproteins of different molecular weights. Lectin binding studies on nuclear matrix fractions revealed the presence of galactose, fucose, and/or sialic acid residues in proteins. Glycosidase experiments indicated that some but not all of these glycoproteins had N-linked sidechains.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.41298 |
| Date | January 1992 |
| Creators | Hemming, Richard John |
| Contributors | Bennett, Gary (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Anatomy.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001351481, proquestno: NN91835, Theses scanned by UMI/ProQuest. |
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