Regulators of G-protein Signaling (RGSs) are negative regulators of G-protein Coupled Receptor (GPCR) mediated signaling that function to limit the lifetime of receptor-activated Galpha proteins. Heterologously expressed mammalian RGSs can functionally complement a yeast mutant lacking its RGS containing gene SST2. Here we show that four mammalian RGSs differentially inhibit the activation of a FUS1-LacZ reporter gene by the STE2 encoded GPCR in yeast with the apparent rank order potency: RGS1 > RGS16 > RGS2 > RGS5. In order to examine the role of the GPCR in modulating RGS function, we functionally expressed the human somatostatin receptor 5 (SSTR5) in yeast. / The ability of RGSs to inhibit SSTR5 signaling was further assessed in cells expressing modified Gpa1 proteins. / Yeast have also been shown to be a useful model organism for the study of the localization of mammalian RGS proteins. We have constructed a series of vectors that allow us to express proteins fused to a Green Fluorescent Protein (GFP). (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.33013 |
| Date | January 2001 |
| Creators | Kong, Janice, 1978- |
| Contributors | Greenwood, Michael (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Anatomy and Cell Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001838370, proquestno: MQ75327, Theses scanned by UMI/ProQuest. |
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